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dc.contributor.authorHarper Jen
dc.contributor.authorDe Vega Jen
dc.contributor.authorSwain Sen
dc.contributor.authorHeavens Den
dc.contributor.authorGasior Den
dc.contributor.authorThomas Aen
dc.contributor.authorEvans Cen
dc.contributor.authorLovatt Aen
dc.contributor.authorLister Sen
dc.contributor.authorThorogood Den
dc.contributor.authorSkot Len
dc.contributor.authorHegarty Men
dc.contributor.authorBlackmore Ten
dc.contributor.authorKudrna Den
dc.contributor.authorByrne Sen
dc.contributor.authorAsp Ten
dc.contributor.authorPowell Wen
dc.contributor.authorFernandez-Fuentes Nen
dc.contributor.authorArmstead Ien
dc.date.accessioned2019-02-05T09:31:38Z
dc.date.available2019-02-05T09:31:38Z
dc.date.issued2019
dc.identifier.urihttps://doi.org/10.1093/aob/mcy230
dc.identifier.urihttp://hdl.handle.net/11262/11585
dc.description.abstractBackground and Aims Lolium perenne (perennial ryegrass) is the most widely cultivated forage and amenity grass species in temperate areas worldwide and there is a need to understand the genetic architectures of key agricultural traits and crop characteristics that deliver wider environmental services. Our aim was to identify genomic regions associated with agriculturally important traits by integrating a bacterial artificial chromosome (BAC)-based physical map with a genome-wide association study (GWAS). Methods BAC-based physical maps for L. perenne were constructed from ~212 000 high-information-content fingerprints using Fingerprint Contig and Linear Topology Contig software. BAC clones were associated with both BAC-end sequences and a partial minimum tiling path sequence. A panel of 716 L. perenne diploid genotypes from 90 European accessions was assessed in the field over 2 years, and genotyped using a Lolium Infinium SNP array. The GWAS was carried out using a linear mixed model implemented in TASSEL, and extended genomic regions associated with significant markers were identified through integration with the physical map. Key Results Between ~3600 and 7500 physical map contigs were derived, depending on the software and probability thresholds used, and integrated with ~35 k sequenced BAC clones to develop a resource predicted to span the majority of the L. perenne genome. From the GWAS, eight different loci were significantly associated with heading date, plant width, plant biomass and water-soluble carbohydrate accumulation, seven of which could be associated with physical map contigs. This allowed the identification of a number of candidate genes. Conclusions Combining the physical mapping resource with the GWAS has allowed us to extend the search for candidate genes across larger regions of the L. perenne genome and identified a number of interesting gene model annotations. These physical maps will aid in validating future sequence-based assemblies of the L. perenne genome.en
dc.language.isoenen
dc.relation.isformatof15022en
dc.relation.ispartofAnnals of Botanyen
dc.rights© The Author(s) 2019. Published by Oxford University Press on behalf of the Annals of Botany Company. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subjectLolium perenneen
dc.subjectBAC-based physical mapen
dc.subjectFPCen
dc.subjectLTCen
dc.subjectGWASen
dc.subjectEcotypesen
dc.subjectCandidate genesen
dc.subjectFloweringen
dc.subjectHeading dateen
dc.subjectWater-soluble carbohydrateen
dc.subjectPlant widthen
dc.titleIntegrating a newly developed BAC-based physical mapping resource for Lolium perenne with a genome-wide association study across a L. perenne European ecotype collection identifies genomic contexts associated with agriculturally important traitsen
dc.typeArticleen
dc.description.versionAccepted manuscript
rioxxterms.publicationdate2019-02-02
rioxxterms.typeJournal Article/Reviewen
dcterms.dateAccepted2018-11-28
refterms.accessExceptionNAen
refterms.dateDeposit2019-02-05
refterms.depositExceptionpublishedGoldOAen
refterms.depositExceptionExplanationGoldOAen
refterms.panelUnspecifieden
refterms.technicalExceptionNAen
refterms.versionAMen


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