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dc.contributor.authorYang Len
dc.contributor.authorBai Jen
dc.contributor.authorZeng Nen
dc.contributor.authorZhou Xen
dc.contributor.authorLiao Yen
dc.contributor.authorLu Yen
dc.contributor.authorRees RMen
dc.contributor.authorNie Jen
dc.contributor.authorCao Wen
dc.date.accessioned2019-01-09T10:37:04Z
dc.date.available2019-01-09T10:37:04Z
dc.date.issued2018
dc.identifier.citation136
dc.identifier.urihttps://doi.org/10.1016/j.apsoil.2018.12.015
dc.identifier.urihttp://hdl.handle.net/11262/11568
dc.description.abstractThe nitrogen (N)-fixing community in soil, a key functional player to replenish N pools from atmospheric N2, can be radically changed by the addition of inorganic or organic substrate; however, the response of the diazotroph population to nutrient and residue management in green manure-based wetland rice rotations is poorly understood. Here, we investigated the diazotrophic community to inputs of a leguminous green manure Chinese milk vetch (Mv, Astragalus sinicus) using quantitative PCR and Illumina Miseq sequencing of the nifH gene. Five treatments were compared in a Milk vetch–early rice–late rice rotation: 1) Control, no fertilization and straw return (CK); 2) rice straw incorporation alone (Rs); 3) inorganic fertilizer application alone (F); 4) F plus Rs (FRs); 5) similar with FRs but high-stubble (∼35–40 cm) of late rice straw was remained (FRh). The results showed that cultivation practice affected the magnitude of soil available nutrient pools (N, P and K), but not the soil organic matter and total N pools. The rice straw significantly repressed the nifH gene abundance compared to the control, and increased the number of diazotrophic bacteria species, Chao 1 values, Shannon and Simpson indexes more than other fertilization treatments. Multivariate regression tree analysis revealed that the community diversity and structure of diazotrophs were primarily shaped by soil nitrate and available P status, as well as C/N ratio. The most abundant genus Bradyrhizobium (21%–32%) tended to decrease in rice straw soil in comparison with the control but was significantly enhanced in the FRs treatment at seedling stage and in FRh treatment at flowering stage. Spearman correlation analysis showed that the dominant diazotrophic genera were positively related with soil available phosphorus, but responded differentially to soil total N, nitrate, and pH between the seedling and flowering stages. Overall, the planting and incorporation of vetch under different practices of residue and fertilizer management reshaped the diazotrophic community during the green manure season, highlighting the crucial roles of soil C, N, and P status or their ratios in shaping the population and diversity of diazotrophs.en
dc.description.sponsorshipBBSRC grant no.: BB/N013484/1 N-CIRCLEen
dc.language.isoenen
dc.relation.isformatof14998en
dc.relation.ispartofApplied Soil Ecologyen
dc.rightsCopyright © 2018 Elsevier B.V. All rights reserved. This manuscript version is made available after the end of the 12 month embargo period under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectChinese milk vetchen
dc.subjectDiazotrophic communityen
dc.subjectInorganic fertilizeren
dc.subjectnifHen
dc.subjectRiceen
dc.subjectStraw returnen
dc.titleDiazotroph abundance and community structure are reshaped by straw return and mineral fertilizer in rice-rice-green manure rotationen
dc.typeArticleen
dc.description.versionAccepted manuscript
dc.extent.pageNumbers11-20
rioxxterms.publicationdate2018-12-26
rioxxterms.typeJournal Article/Reviewen
dcterms.dateAccepted2018-12-17
refterms.accessExceptionNAen
refterms.dateDeposit2019-01-09
refterms.dateEmbargoEnd2019-12-26
refterms.dateFreeToDownload2019-12-26
refterms.dateFreeToRead2019-12-26
refterms.dateToSearch2019-12-26
refterms.depositExceptionNAen
refterms.panelUnspecifieden
refterms.technicalExceptionNAen
refterms.versionAMen


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