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dc.contributor.authorStewart RDen
dc.contributor.authorAuffret MDen
dc.contributor.authorWarr Aen
dc.contributor.authorWiser AHen
dc.contributor.authorPress MOen
dc.contributor.authorLangford KWen
dc.contributor.authorLiachko Ien
dc.contributor.authorSnelling TJen
dc.contributor.authorDewhurst RJen
dc.contributor.authorWalker AWen
dc.contributor.authorRoehe Ren
dc.contributor.authorWatson Men
dc.date.accessioned2018-07-17T10:42:39Z
dc.date.available2018-07-17T10:42:39Z
dc.date.issued2018
dc.identifier.citation9en
dc.identifier.urihttp://hdl.handle.net/11262/11481
dc.identifier.urihttps://doi.org/10.1038/s41467-018-03317-6
dc.description.abstractThe cow rumen is adapted for the breakdown of plant material into energy and nutrients, a task largely performed by enzymes encoded by the rumen microbiome. Here we present 913 draft bacterial and archaeal genomes assembled from over 800 Gb of rumen metagenomic sequence data derived from 43 Scottish cattle, using both metagenomic binning and Hi-Cbased proximity-guided assembly. Most of these genomes represent previously unsequenced strains and species. The draft genomes contain over 69,000 proteins predicted to be involved in carbohydrate metabolism, over 90% of which do not have a good match in public databases. Inclusion of the 913 genomes presented here improves metagenomic read classification by sevenfold against our own data, and by fivefold against other publicly available rumen datasets. Thus, our dataset substantially improves the coverage of rumen microbial genomes in the public databases and represents a valuable resource for biomass-degrading enzyme discovery and studies of the rumen microbiome.en
dc.description.sponsorshipBBSRC grant no. BB/N0172X/1en
dc.language.isoenen
dc.relation.isformatof14893en
dc.relation.ispartofNature Communicationsen
dc.rights© The Author(s) 2018 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.subjectArchaeal genomicsen
dc.subjectBacterial genomicsen
dc.subjectEnvironmental microbiologyen
dc.subjectMetagenomicsen
dc.titleAssembly of 913 microbial genomes from metagenomic sequencing of the cow rumenen
dc.typeArticleen
dc.description.versionVersion of Record
dc.extent.pageNumbersarticle 870en
rioxxterms.publicationdate2018-02-28
rioxxterms.typeJournal Article/Reviewen
dcterms.dateAccepted2018-02-05
refterms.accessExceptionNAen
refterms.dateDeposit2018-07-17
refterms.depositExceptionpublishedGoldOAen
refterms.depositExceptionExplanationGoldOAen
refterms.panelUnspecifieden
refterms.technicalExceptionconferenceOutsideDefinitionen
refterms.versionVoRen


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