Show simple item record

dc.contributor.authorDussart Fen
dc.contributor.authorDouglas Ren
dc.contributor.authorSjokvist Een
dc.contributor.authorHoebe PNen
dc.contributor.authorSpoel SHen
dc.contributor.authorMcGrann GRDen
dc.date.accessioned2018-05-09T10:54:52Z
dc.date.available2018-05-09T10:54:52Z
dc.date.issued2018
dc.identifier.citation31:9
dc.identifier.urihttps://doi.org/10.1094/MPMI-12-17-0299-R
dc.identifier.urihttp://hdl.handle.net/11262/11440
dc.description.abstractRamularia collo-cygni (Rcc) causes Ramularia leaf spot (RLS) disease of barley. The fungus develops asymptomatically within its host until late in the growing season when necrotic lesions become visible on upper leaves. Fungal secondary metabolites (SMs) have been proposed as important factors in RLS lesion formation, but the biosynthetic pathways involved remain largely unknown. Mining the Rcc genome revealed the presence of ten polyketide synthases (PKSs), ten non-ribosomal peptide synthetases (NRPSs) and three hybrid PKS-NRPS (HPSs) identified within clusters of genes with predicted functions associated with secondary metabolism. SM core genes along with their predicted transcriptional regulators exhibited transcriptional co-expression during infection of barley plants. Moreover, their expression peaked during early stages of host colonisation and preceded or overlapped with the appearance of disease symptoms, suggesting that SMs may manipulate the host to promote colonisation or protect Rcc from competing organisms. Accordingly, Rcc inhibited the growth of several fungi in vitro, indicating it synthesised and excreted anti-fungal agents. Taken together, these findings demonstrate that the Rcc genome contains the genetic architecture to synthesize a wide range of SMs and suggests that co-expression of PKSs and HPSs is associated with competitive colonisation of the host and early symptom development.en
dc.description.sponsorshipScottish Government RESAS Strategic Research Programme (RD2.1.3)en
dc.language.isoenen
dc.relation.isformatof14799en
dc.relation.ispartofMolecular Plant-Microbe Interactionsen
dc.rightsCopyright:The American Phytopathological Society https://doi.org/10.1094/MPMI-12-17-0299-R
dc.titleGenome-based discovery of polyketide-derived secondary metabolism pathways in the barley pathogen Ramularia collo-cygnien
dc.typeArticleen
dc.description.versionAccepted manuscript
dc.extent.pageNumbers962-975
rioxxterms.publicationdate2018-03-21
rioxxterms.publicationdate2018-07-20
rioxxterms.typeJournal Article/Reviewen
dcterms.dateAccepted2018-03-21
refterms.accessExceptionNAen
refterms.dateDeposit2018-04-04
refterms.depositExceptionNAen
refterms.depositExceptionIt would be unlawful to deposit, or request deposit of, the output.
refterms.depositExceptionExplanationPermission was not sought by the author prior to publication, which would have allowed arching of the pre-print version.
refterms.panelUnspecifieden
refterms.technicalExceptionNAen
refterms.versionAMen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record