Investigation into the effects of 6-Benzylaminopurine and 1-Naphthaleneacetic acid concentrations on 3 micropropagated Begonia rex 'Fedor' explants
Developing standardised tissue culture techniques for Begonia cultivars would assist botanical organisations in maintaining collections and enable sharing of plants worldwide. Currently there are no published papers on Begonia rex ‘Fedor’ regeneration in tissue culture. This project investigates the effects of 1- Naphthaleneacetic acid (NAA) and 6-Benzylaminopurine (BA) on three B. rex ‘Fedor’ explant types (lamina mid rib, petiole and leaf lamina) In Vitro. Relevant tissue cultures were placed in four varying concentrations of combined NAA and BA (0.70 μM and 0.60 μM, 0.55 μM and 0.98 μM, 0.20 μM and 0.26 μM, 0.28 μM and 0.49 μM) in Murashige and Skoog (1962) (Murashige and Skoog, 1962) medium. Explants were placed in optimum conditions (23.0°C ± 1.5°C with photoperiod of 16-hour light to 8-hour dark) for eight weeks and periodically monitored for root and vegetative expansion. The recorded data included multiple variables, therefore, a two-way ANOVA statistical test with replication was applied. Results found that leaf lamina explants placed in NAA 0.55 μM and BA 0.98 μM had the highest instance of leaf regeneration (2.7 per plant). The longest mean root length (2.24 cm) was observed in explants placed in 0.28 μM NAA and 0.49 μM BA. The lamina mid rib explants placed in 0.7 μM NAA and 0.6 μM BA gained the highest amount of fresh weight (0.52 g). The longest vegetative expansion was leaf lamina explants placed in 0.7 μM NAA and 0.6 μM BA. Further investigation for this project could include trialling other plant organs, such as the apical tip. Future re-trials could confirm that results are representative and accurate.
Other Titles/Title of Conference
International Forum - Agriculture, Biology and Life Science (IFABL 2016), Kurume, Japan
Journal Title/Title of Proceedings
Proceedings of the 2016 International Forum – Agriculture, Biology, and Life Science.