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dc.contributor.authorDeasy Wen
dc.contributor.authorShepherd Ten
dc.contributor.authorAlexander CJen
dc.contributor.authorBirch ANEen
dc.contributor.authorEvans KAen
dc.date.accessioned2016-10-31T17:04:17Z
dc.date.available2016-10-31T17:04:17Z
dc.date.issued2016
dc.identifier.citation27:6en
dc.identifier.issn0958-0344
dc.identifier.urihttp://dx.doi.org/10.1002/pca.2634
dc.identifier.urihttp://hdl.handle.net/11262/11119
dc.description.abstractIntroduction – Collection of volatiles from plant roots poses technical challenges due to difficulties accessing the soil environment without damaging the roots. Objectives – To validate a new non-invasive method for passive sampling of root volatiles in situ, from plants grown under field conditions, using solid phase micro-extraction (SPME). Methods – SPME fibres were inserted into perforated polytetrafluoroethene (PTFE) tubes positioned in the soil next to broccoli plants for collection of root volatiles pre- and post-infestation with Delia radicum larvae. After sample analysis by gas chromatography-mass spectrometry (GC-MS), principal component analysis (PCA) was applied to determine differences in the profiles of volatiles between samples. Results – GC-MS analysis revealed that this method can detect temporal changes in root volatiles emitted before and after Delia radicumdamage. PCA showed that samples collected pre- and post-infestationwere compositionally different due to the presence of root volatiles induced by D. radicum feeding. Sulphur containing compounds, in particular, accounted for the differences observed. Root volatiles emission patterns post-infestation are thought to follow the feeding and developmental progress of larvae. Conclusion – This study shows that volatiles released by broccoli roots can be collected in situ using SPME fibres within perforated PTFE tubes under field conditions. Plants damaged by Delia radicum larvae could be distinguished from plants sampled preinfestation and soil controls on the basis of larval feeding-induced sulphur-containing volatiles. These results show that this newmethod is a powerful tool for non-invasive sampling of root volatiles below-ground.en
dc.language.isoenen
dc.relation.isformatof14470en
dc.relation.ispartofPhytochemical Analysisen
dc.rightsCopyright © 2016 John Wiley & Sons, Ltd. This is the accepted version of the above article, which has been published in final form at http://dx.doi.org/10.1002/pca.2634
dc.subjectBrassicaen
dc.subjectDelia radicumen
dc.subjectField and soilen
dc.subjectSPME-GC-MSen
dc.subjectIn situ root volatiles analysisen
dc.titleField-based evaluation of a novel SPME-GC-MS method for investigation of below-ground interaction between brassica roots and larvae of cabbage root fly, Delia radicum Len
dc.typeArticleen
dc.description.versionAccepted manuscript
dc.extent.pageNumbers343-353en
rioxxterms.publicationdate2016-09-30
rioxxterms.typeJournal Article/Reviewen
dcterms.dateAccepted2016-05-19
refterms.accessExceptionNAen
refterms.dateDeposit2016-10-31
refterms.dateEmbargoEnd2017-10-31
refterms.depositExceptionNAen
refterms.panelUnspecifieden
refterms.technicalExceptionNAen
refterms.versionAMen


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