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dc.contributor.authorChintoa-Uta Cen
dc.contributor.authorCassady-Cain RLen
dc.contributor.authorAl-Haideri Hen
dc.contributor.authorWatson Een
dc.contributor.authorKelly DJen
dc.contributor.authorSmith DGEen
dc.contributor.authorSparks NHCen
dc.contributor.authorKaiser Pen
dc.contributor.authorStevens MPen
dc.date.accessioned2016-04-29T11:20:26Z
dc.date.available2016-04-29T11:20:26Z
dc.date.issued2015
dc.identifier.citation33:46en
dc.identifier.urihttp://dx.doi.org/10.1016/j.vaccine.2015.09.100
dc.identifier.urihttp://hdl.handle.net/11262/10990
dc.description.abstractCampylobacter is the leading cause of foodborne diarrhoeal illness in the developed world and consump-tion or handling of contaminated poultry meat is the principal source of infection. Strategies to controlCampylobacter in broilers prior to slaughter are urgently required and are predicted to limit the inci-dence of human campylobacteriosis. Towards this aim, a purified recombinant subunit vaccine basedon the superoxide dismutase (SodB) protein of C. jejuni M1 was developed and tested in White Leghornbirds. Birds were vaccinated on the day of hatch and 14 days later with SodB fused to glutathione S-transferase (GST) or purified GST alone. Birds were challenged with C. jejuni M1 at 28 days of age andcaecal Campylobacter counts determined at weekly intervals. Across three independent trials, the vaccineinduced a statistically significant 1 log10reduction in caecal Campylobacter numbers in vaccinated birdscompared to age-matched GST-vaccinated controls. Significant induction of antigen-specific serum IgYwas detected in all vaccinated birds, however the magnitude and timing of SodB-specific IgY did not cor-relate with lower numbers of C. jejuni. Antibodies from SodB-vaccinated chickens detected the proteinin the periplasm and not membrane fractions or on the bacterial surface, suggesting that the protectionobserved may not be strictly antibody-mediated. SodB may be useful as a constituent of vaccines forcontrol of C. jejuni infection in broiler birds, however modest protection was observed late relative to thelife of broiler birds and further studies are required to potentiate the magnitude and timing of protection.en
dc.description.sponsorshipBBSRC- BB/J500537/1 and BB/J004227/1en
dc.language.isoenen
dc.relation.isformatof14295en
dc.relation.ispartofVaccineen
dc.rightsCopyright © 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
dc.subjectCampylobacter jejunien
dc.subjectChickensen
dc.subjectVaccineen
dc.subjectSuperoxide dismutaseen
dc.subjectAntibodyen
dc.subjectProtectionen
dc.titleSuperoxide dismutase SodB is a protective antigen against Campylobacter jejuni colonisation in chickensen
dc.typeArticleen
dc.description.versionVersion of record
dc.extent.pageNumbers6206-6211en
rioxxterms.publicationdate2015-11-17
rioxxterms.typeJournal Article/Reviewen
dcterms.dateAccepted2015-09-29
refterms.accessExceptionNAen
refterms.dateDeposit2016-04-29
refterms.depositExceptionpublishedGoldOAen
refterms.panelUnspecifieden
refterms.technicalExceptionNAen
refterms.versionVoRen


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